Anti-inflammatory effect of 4-O-methylhonokiol, a novel compound isolated from Magnolia officinalis through inhibition of NF-kappa B

作者：Oh Ju Hoon; Kang La La; Ban Jung Ok; Kim Young Heui; Kim Ki Ho; Han Sang Bae; Hong Jin Tae^*

来源：Chemico-Biological Interactions, 2009, 180(3): 506-514.

DOI：10.1016/j.cbi.2009.03.014

• 4-O-methylhonokiol
• NF-kappa B
• NO
• Anti-inflammation

The bioactive constituents isolated from the bark of Magnolia officinalis such as magnolol. honokiol and obovatol have anti-inflammatory properties through the inactivation of NF-kappa B which is an important factor in the regulation of inflammatory reaction. We recently isolated a novel neolignan compound, 4-O-methylhonokiol, from M. officinalis. In the present study, we investigated whether or not 4-O-methylhonokiol, inhibits inflammatory reaction through the inhibition of NF-kappa B activity. The results showed that 4-O-methylhonokiol (2.5-10 mu M) inhibited LPS (1 mu g/ml)-induced NO generation in macrophage Raw 264.7 cells in a concentration-dependent manner with IC(50) value 9.8 mu M. The inhibition of NO generation by 4-O-methylhonokiol was consistent with the inhibitory effect on the expression as well as transcriptional activity of inducible nitric oxide synthase (iNOS). In addition, 4-O-methylhonokiol inhibited the LPS-induced transcriptional and DNA binding activities of NF-kappa B as well as p50 and p65 translocation into the nucleus. Topical application of 4-O-methylhonokiol (0.1-1 mg/ear) inhibited 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory ear edema formation, NF-kappa B activity, and iNOS and COX-2 expression. The present results provided evidence that 4-O-methylhonokiol has anti-inflammatory properties through inhibition of the NF-kappa B pathway, and suggested that 4-O-methylhonokiol can be used as an anti-inflammatory agent.

• 出版日期2009-8-14