Downregulated expression of Dicer1 predicts inferior survival in primary gastrointestinal diffuse large B-cell lymphoma treated with CHOP-like regimen and rituximab

作者：Zhao, Haifeng; Zhang, Le; Guo, Shanqi; Yuan, Tian; Xia, Bing; Qu, FuLian; Zhang, Lianyu; Zhang, Yizhuo^*

来源：Medical Oncology, 2014, 31(10): 206.

DOI：10.1007/s12032-014-0206-2

• Gastrointestinal lymphoma
• Diffuse large B-cell lymphoma
• Dicer1
• Immunophenotype
• Prognosis

The aim of this study was to detect the expression levels of Dicer1, Drosha, DGCR8, and Ago2 messenger ribonucleic acids (mRNAs) in patients with primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL) and determine their associations with clinical parameters and prognostic significance. The mRNA level expressions of Dicer1, Drosha, DGCR8, and Ago2 were detected by real-time quantitative polymerase chain reaction. Immunohistochemical staining of CD10, BCL6, and MUM1 was performed using EnVision (TM) system. The clinicopathologic features and follow-up data were analyzed using Kaplan-Meier estimator. The results show that the expression of Dicer1 (P = 0.001), Drosha (P = 0.01), DGCR8 (P = 0.02), and Ago2 (P = 0.002) mRNAs in cancer tissues of patients with PGI-DLBCL was significantly lower than those in normal tissues of healthy controls. Among the expression of CD10, BCL6, and MUM1, 27.4 % (17/62) of the patients belonged to the germinal center B-cell (GCB) subtype and 72.6 % (45/62) belonged to the non-GCB subtype. Dicer1 expression was significantly decreased in the non-GCB subgroup (P = 0.02) and in the high International Prognostic Index (3-5 score) subgroup (P = 0.03). Kaplan-Meier analysis showed that the low-Dicer1 subgroup had a shorter overall survival (P = 0.02) and shorter progression-free survival (P = 0.015) than the high-Dicer1 subgroup. Multivariate analysis identified Dicer1 as an independent prognostic factor in PGI-DLBCL. In Conclusion, Dicer1, Drosha, DGCR8, and Ago2 play key roles in the pathogenesis of PGI-DLBCL. Dicer1, an independent prognostic factor for predicting shortened survival of patients with PGI-DLBCL, can be used as a biomarker to guide the prognosis.

• 出版日期2014-10
• 单位天津医科大学