Association between polymorphisms of interleukin 10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis

作者：Ge Ling; Huang Yong; Zhang Hui; Liu Ruiping^*; Xu Nanwei

来源：Joint Bone Spine, 2015, 82(3): 182-186.

DOI：10.1016/j.jbspin.2014.11.007

• Interleukin 10
• Polymorphisms
• Rheumatoid arthritis
• Molecular epidemiology

Objectives: The aim of the present study was to examine the association between polymorphisms of IL-10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis (RA).
Methods: We examined IL-10 rs1800872 A/C polymorphisms in 615 RA patients, and 839 controls, in an East Chinese Han population. Genotyping was performed using a custom-by-design 48-Plex SNP scan TM Kit. The blood plasma concentration of IL-10 was measured using an Iodine [1251] IL-10 Radioimmunoassay Kit, in 90 RA patients and 90 controls.
Results: IL-10 rs1800872 A/C polymorphisms were associated with risk of RA. Following stratified analysis, an increased risk of RA was associated with the CC genotype among male, older, C-reactive protein-positive, anti-cyclic citrullinated peptide antibody-positive, and rheumatoid factor-positive-patients, and among patients with a DAS28 of >= 3.20 or an erythrocyte sedimentation rate of >= 25, and in functional class land II patients. The average plasma concentration of IL-10 was significantly higher in RA patients compared with controls. RA patients positive for the homozygote CC were characterized by significantly higher levels of IL-10 compared with patients with the heterozygote AC. We also found that there were significant relationships between the single nucleotide polymorphisms in the human IL-10 rs1800872 A/C and production of IL-10.
Conclusions: Our results suggest that the IL-10 rs1800872 A/C allele might increase the risk of RA. The IL-10 rs1800872 A/C allele might also impact the inflammatory reaction of IL-10 in patients with RA.

• 出版日期2015-5
• 单位南京医科大学