An ETHYLENE RESPONSE FACTOR-MYB Transcription Complex Regulates Furaneol Biosynthesis by Activating QUINONE OXIDOREDUCTASE Expression in Strawberry

作者：Zhang, Yuanyuan; Yin, Xueren; Xiao, Yuwei; Zhang, Zuying; Li, Shaojia; Liu, Xiaofen; Zhang, Bo; Yang, Xiaofang; Grierson, Donald; Jiang, Guihua; Klee, Harry J.; Chen, Kunsong^*

来源：Plant Physiology, 2018, 178(1): 189-201.

DOI：10.1104/pp.18.00598

• FRAGARIA X ANANASSA
• AROMA COMPOUNDS
• FRAGRANCE BIOSYNTHESIS
• EUGENOL PRODUCTION
• FLAVOR COMPOUND
• FRUIT
• GENES
• 4-HYDROXY-2,5-DIMETHYL-3(2H)-FURANONE
• 2,5-DIMETHYL-4-HYDROXY-3(2H)-FURANONE
• AGROINFILTRATION

4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is a major contributor to the aroma of strawberry (Fragaria x ananassa) fruit, and the last step in its biosynthesis is catalysed by Fragaria x ananassa quinone oxidoreductase (FaQR). Here, an ethylene response factor (FaERF#9) was characterized as a positive regulator of the FaQR promoter. Linear regression analysis indicated that FaERF#9 transcript levels were significantly correlated with both FaQR transcripts and furanone content in different strawberry cultivars. Transient overexpression of FaERF#9 in strawberry fruit significantly increased FaQR expression and furaneol production. Yeast one-hybrid assays, however, indicated that FaERF#9 by itself did not bind to the FaQR promoter. An MYB transcription factor (FaMYB98) identified in yeast one-hybrid screening of the strawberry cDNA library was capable of both binding to the promoter and activating the transcription of FaQR by similar to 5.6-fold. Yeast two-hybrid assay and bimolecular fluorescence complementation confirmed a direct protein-protein interaction between FaERF#9 and FaMYB98, and in combination, they activated the FaQR promoter 14-fold in transactivation assays. These results indicate that an ERF-MYB complex containing FaERF#9 and FaMYB98 activates the FaQR promoter and upregulates HDMF biosynthesis in strawberry.

• 出版日期2018-9
• 单位浙江大学; 浙江省农业科学院