Development and application of a droplet digital polymerase chain reaction (ddPCR) for detection and investigation of African swine fever virus

作者：Wu, Xulong; Xiao, Lu; Lin, Hua; Chen, Shijie; Yang, Miao; An, Wei; Wang, Yin^*; Yang, Zexiao; Yao, Xueping; Tang, Zizhong

来源：CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2018, 82(1): 70-74.

• PCR

The aim of this study was to develop a droplet digital polymerase chain reaction (ddPCR) method to detect African swine fever virus (ASFV). The methods of ASFV real-time PCR and ddPCR were established and optimal reaction conditions were confirmed. Each method was evaluated for linearity, limit of detection, and specificity. The results indicated that ASFV ddPCR had a high degree of linearity (R-2 >= 0.998) and specificity. The detection limit was 10 copies/reaction, which was approximately a 10-fold greater sensitivity than real-time PCR. This sensitive method could be used as an efficient molecular biology tool to diagnose ASFV, which is very important for preventing the spread of diseases across borders.

• 出版日期2018-1
• 单位四川省畜牧科学研究院; 四川省农业科学院; 四川农业大学