A novel pancreatic beta-cell targeting bispecific-antibody (BsAb) can prevent the development of Type 1 diabetes in NOD mice

作者:Bhattacharya Palash; Fan Jilao; Haddad Christine; Essani Abdul; Gopisetty Anupama; Elshabrawy Hatem A; Vasu Chenthamarakshan; Prabhakar Bellur S*
来源:Clinical Immunology, 2014, 153(1): 187-198.
DOI:10.1016/j.clim.2014.04.014

摘要

To prepare a novel Bispecific Antibody (BsAb) as a potential targeted therapy for T1D, we produced a %26quot;functionally inert%26quot; monoclonal antibody (mAb) against Glucose transporter-2 (GLUT-2) expressed on beta-cells to serve as an anchoring antibody. The therapeutic am is. an agonistic mAb against Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), a negative regulator of T-cell activation expressed on activated CD4+ T-cells. A BsAb was prepared by chemically coupling an anti-GLUT2 mAb to an agonistic anti-CTLA-4 mAb. This BsAb was able to bind to GLUT2 and CTLA-4 in vitro, and to pancreatic islets, both in vitro and in vivo. We tested the safety and efficacy of this BsAb by treating Non-Obese Diabetes (NOD) mice and found that it could delay the onset of diabetes with no apparent undesirable side effects. Thus, engagement of CTLA-4 on activated T cells from target tissue can be an effective way to treat type-1 diabetes.

  • 出版日期2014-7