Asymmetric synthesis of (R)-mandelonitrile catalysed by (R)-oxynitrilase from almond in water/organic solvent biphase systems

作者：Liu SL; Zong MH^*; Tu R; Zhou F

来源：Chinese Journal of Catalysis, 2001, 22(1): 67-70.

• benzaldehyde
• hydrogen cyanide
• asymmetric synthesis
• oxynitrilase
• (R)-mandelonitrile

The asymmetric synthesis of (R)-mandelonitrile from benzaldehyde and hydrogen cyanide using (R)-oxynitrilase from bitter almond in water/organic solvent biphase systems was studied. The effects of organic solvents, volume ratio of water phase to organic phase, pH value of aqueous phase and reaction temperature on the initial reaction rate, the final conversion and enantiomeric purity of the product were explored. All above mentioned facts had important effects on the reaction. Diisopropyl ether is found to be the best organic solvent for the reaction. The optimum volume ratio of water phase to organic phase, pH and reaction temperature are 1/2, 3.4 and 0 similar to 5 degreesC respectively for the reaction. Under these conditions, both the conversion rate and enantiomeric excess are as high as 99%.

• 出版日期2001-1
• 单位华南理工大学