A Selective Cell Population from Dermis Strengthens Bone Regeneration

作者：Wang, Tingliang; He, Jinguang; Zhang, Yang; Shi, Wenjun; Dong, Jiasheng; Pei, Ming^*; Zhu, Lian^*

来源：Stem Cells Translational Medicine, 2017, 6(1): 306-315.

DOI：10.5966/sctm.2015-0426

• Skin
• Bone morphogenetic protein receptor type IB
• Bone tissue engineering
• Coral
• Calvarial defect

Finding appropriate seed cells for bone tissue engineering remains a significant challenge. Considering that skin is the largest organ, wehypothesized that human bone morphogenetic protein receptor type IB (BmprIB)+ dermal cells could have enhanced osteogenic capacity in the healing of critical-sized calvarial defects in an immunodeficient mouse model. In this study, immunohistochemical staining revealed that BmprIB was expressed throughout reticular dermal cells; the positive expression rate of BmprIB was 3.5% +/- 60.4% in freshly separated dermal cells, by flow cytometry. Furthermore, in vitro osteogenic capacity of BmprIB+ cells was confirmed by osteogenic-related staining and marker gene expression compared with unsorted dermal cells. In vivo osteogenic capacity was demonstrated by implantation of human BmprIB+ cell/coral constructs in the treatment of 4-mm diameter calvarial defects in an immunodeficient mouse model compared with implantation of unsorted cell/coral constructs and coral scaffold alone. These results indicate that the selective cell population BmprIB from human dermis is a promising osteogenic progenitor cell that can be a large-quantity and high-quality cell source for bone tissue engineering and regeneration.

• 出版日期2017-1
• 单位上海交通大学