Multiplexing analysis of the polyspecific intrathecal immune response in multiple sclerosis

作者：Kulakowska Alina; Mroczko Barbara; Mantur Maria; Lelental Natalia; Tarasiuk Joanna; Kapica Topczewska Katarzyna; Schulz Ute; Lange Peter; Zimmermann Ruediger; Kornhuber Johannes; Lewczuk Piotr^*

来源：Methods, 2012, 56(4): 528-531.

DOI：10.1016/j.ymeth.2012.03.002

• Multiple sclerosis
• Clinical neurochemistry
• Cerebrospinal fluid
• MRZH-reaction
• Multiplexing
• Neuroimmunology

Intrathecal synthesis of the antibodies specific to neurotrofic viruses: measles (M), rubella (R), Varicella-Zoster (Z), and/or H. simplex (H), known as %26quot;MRZH-reaction%26quot; plays important diagnostic role in multiple sclerosis (MS). Whereas the analysis of the oligoclonal IgG bands provides high sensitivity, the MRZH-reaction shows high specificity, and hence these methods complement each other. For the first time we applied multiplexing bead-based technology to simultaneously analyze cerebrospinal fluid (CSF) and serum concentrations of antibodies against these viruses, and to calculate the antibody specific indices (ASI%26apos;s). The method shows reasonable precision: intra-assay, 2.9-6.7%, and inter-assay, 2.0-3.2%. The results are comparable with these obtained with other methods (ELISAs), including two runs of the certified external quality control schemes. Eighty-one percent of the MS cases (n = 27) and none of the sex- and age-matched controls (n = 14), except one subject with %26quot;borderline%26quot; anti-measles ASI of 1.5, showed intrathecal synthesis of IgG against at least one of the viruses discussed. The ratios of the MRZH-positive cases in the MS group were: 12/22 for M, 12/19 for R, 13/26 for Z, and 7/26 for H. We conclude that the multiplexing technology can be applied as a tool to study the intrathecal immune response in the diagnosis of MS.

• 出版日期2012-4