Protective effects of grape seed extract against oxidative and nitrative damage of plasma proteins

作者：Bijak Michal^*; Kolodziejczyk Czepas Joanna; Ponczek Michal Blazej; Saluk Joanna; Nowak Pawel

来源：International Journal of Biological Macromolecules, 2012, 51(3): 183-187.

DOI：10.1016/j.ijbiomac.2012.05.009

• Grape seeds
• Antioxidant
• Catechins
• Carbonyl groups
• 3-Nitrotyrosine

Oxidative stress, vascular inflammation, endothelial dysfunction plays a crucial role in the pathogenesis of cardiovascular diseases.
The aim of our in vitro study was to examine the antioxidative properties of grape seed extract, and its potential protective effect on the haemostatic function of human fibrinogen under oxidative stress conditions, induced by peroxynitrite (100 mu M).
The preincubation of plasma with the tested extract (0.5-50 mu g/ml or 0.5-300 mu g/ml) reduced the formation of 3-nitrotyrosine and diminished oxidation of thiol groups in plasma proteins. The low concentrations (0.5-50 mu g/ml) of grape seed extract also decreased the level of carbonyl groups, however at higher concentrations (100-300 mu g/ml) this effect was not observed. Furthermore, grape seed extract counteracted the inhibitory effect of peroxynitrite on human plasma clotting.
The results obtained in this study indicate that components of the grape seed extract posses antioxdative properties and may be promising substances for the creation of new dietary supplements.

• 出版日期2012-10