Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

作者：Won M; Park K A; Byun H S; Sohn K C; Kim Y R; Jeon J; Hong J H; Park J; Seok J H; Kim J M; Yoon W H; Jang I S; Shen H M; Liu Z G; Hur G M^*

来源：Cell Death and Differentiation, 2010, 17(12): 1830-1841.

DOI：10.1038/cdd.2010.47

• TNF receptor
• JNK
• NF-kappa B
• apoptosis
• cellular signaling

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-kappa B (NF-kappa B) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-kappa B function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-kappa B activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis. Cell Death and Differentiation (2010) 17, 1830-1841; doi:10.1038/cdd.2010.47; published online 7 May 2010

• 出版日期2010-12
• 单位河北医科大学