A Comparative Fluorescent Beacon-based Method for Serum microRNA Quantification

作者：Beta Madhu; Krishnakumar Subramanian; Elchuri Sailaja V; Salim Bindu; Narayanan Janakiraman^*

来源：Analytical Sciences, 2015, 31(3): 231-235.

DOI：10.2116/analsci.31.231

• Locked nucleic acid (LNA)
• serum
• miRNA
• retinoblastoma

Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 mu L healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.

• 出版日期2015-3