Simultaneous ultrasound-assisted water extraction and beta-cyclodextrin encapsulation of polyphenols from Mangifera indica stem bark in counteracting TNFa-induced endothelial dysfunction

作者：Mura Marzia^*; Palmieri Daniela; Garella Davide; Di Stilo Antonella; Perego Patrizia; Cravotto Giancarlo; Palombo Domenico

来源：Natural Product Research, 2015, 29(17): 1657-1663.

DOI：10.1080/14786419.2014.996753

• beta-cyclodextrin
• ultrasound-assisted extraction
• Mangifera indica
• endothelial dysfunction
• TNF alpha
• oestrogen receptor

This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of -cyclodextrin (-CD) at room temperature and testing their biological activity on TNF-induced endothelial dysfunction. Both extracts counteracted TNF effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. -CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the -CD extract induced Akt activation and completely abolished the TNF-induced p38MAPK phosphorylation. UAE and -CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.

• 出版日期2015-9-2