Differential pro-apoptotic effect of allicin in oestrogen receptor-positive or -negative human breast cancer cells

作者：Kim Kyung Ho; Cho Seong Jun; Kim Byung Oh; Pyo Suhkneung^*

来源：Journal of Functional Foods, 2016, 25: 341-353.

DOI：10.1016/j.jff.2016.06.019

• Allicin
• Apoptosis
• Oestrogen receptor
• Breast cancer
• ER
• MAPK

Allicin is known to induce apoptosis and inhibit tumourigenesis in various carcinoma cells. However, the precise mechanism of allicin-induced apoptosis remains unclear in human breast cancer cells. Here we found that ER alpha-MDA-MB-231 cells were more sensitive to allicin-induced apoptosis as compared with ER alpha(+)MCF7 cells. We also found that allicin induced reactive oxygens species (ROS)-mediated and caspase-dependent apoptosis in MDA-MB-231 cells, but not in MCF7 cells. Additionally, we showed the p38 and JNK pathways were involved in allicin-induced apoptosis. Furthermore, we demonstrated that ER alpha-knockdown increased cell growth suppression and apoptosis of MCF7 cells in response to allicin, whereas ER alpha-overexpression decreased cell growth suppression and apoptosis of MDA-MB-231 cells, implicating that ER alpha has a protective role during allicin-induced apoptosis. Collectively, these findings suggest that allicin induces differential apoptotic pathways through MAPK activated by ROS-dependent or -independent signalling pathway in breast cancer cells.

• 出版日期2016-8