Alternative extraction technique for the quantitative analysis of succinic acid in a chitosan-succinic acid powder mixture using GC-MS

作者：Lee Do Gyeong; Park Ji Hae; Yun Jee Hye; Song Myung Chong; Ahn Eun Mi; Chung In Sik; Baek Nam In^*

来源：Food Science and Biotechnology, 2011, 20(6): 1609-1614.

DOI：10.1007/s10068-011-0222-3

• alkaline hydrolysis
• chitosan
• GC-MS
• succinic acid
• validation

Typical solvent extraction of succinic acid from a chitosan-succinic acid powder mixture gave unsatisfactory yield (10.0%) due to the presence of intramolecular crosslinks between the chitosan amine groups and succinic acid carboxylic groups. An improved extraction method, which included hydrolysis of cross-links by KOH to liberate succinic acid from the matrix, afforded satisfactory yield (95.2 +/- 4.0%). Quantitative analysis of succinic acid in chitosan-succinic acid mixture was performed using GCMS and the optimized analysis method showed good validation in terms of specificity, linearity, accuracy (recovery rate), precision (inter-day and intra-day precision), limit of quantification (LOQ), and limit of detection (LOD).

• 出版日期2011-12