Acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of a cellulosic exopolysaccharide obtained from sugarcane molasses

作者：Morono Pinto Flavia Cristina; De Oliveira Ana Cecilia A X; De Carvalho Rosangela R; Gomes Carneiro Maria Regina; Coelho Deise R; Lima Salvador Vilar C; Paumgartten Francisco Jose R; Aguiar Jose Lamartine A

来源：Carbohydrate Polymers, 2016, 137: 556-560.

DOI：10.1016/j.carbpol.2015.10.071

• Antigenotoxicity
• Bacterial Cellulose
• Biomaterial
• Cytotoxicity
• Exopolysaccharide
• Genotoxicity

The acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of BC were studied. Cytotoxicity of BC was evaluated in cultured C3A hepatoma cells (HepG2/C3A) using a lactate dehydrogenase (LDH) activity assay. Acute toxicity was tested in adults Wistar rats treated with a single dose of BC. The genotoxicity of BC was evaluated in vivo by the micronucleus assay. BC (0.33-170 tig/mL) added to C3A cell culture medium caused no elevation in LDH release over the background level recorded in untreated cell wells. The treatment with the BC in a single oral dose (2000 mg/kg body weight) caused no deaths or signs of toxicity. BC attenuated CP-induced and inhibition the incidence of MNPCE (female: 46.94%; male: 22.7%) and increased the ratio of PCE/NCE (female: 46.10%; male: 35.25%). There was no alteration in the LDH release in the wells where C3A cells were treated with increasing concentrations of BC compared to the wells where the cells received the cell culture medium only (background of approximately 20% cell death), indicated that in the dose range tested BC was not cytotoxic. BC was not cytotoxic, genotoxic or acutely toxic. BC attenuated CP-induced genotoxic and myelotoxic effects.

• 出版日期2016-2-10