Antioxidant and Cytotoxic Activities of the Essential Oil and Extracts of Anisosciadium orientale

作者：Masroorbabanari Mehdi; Miri Ramin; Firuzi Omidreza; Jas**i Amir Reza

来源：Journal of the Chemical Society of Pakistan, 2014, 36(3): 457-461.

• Anisosciadium orientale
• Cytotoxic
• Antioxidant

The essential oil of Anisosciadium orientale was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The essential oil (EO) was rich in monoterpene hydrocarbons: terpinolene (19.7 +/- 2.8%, 226.4 +/- 27.6 mg/ g EO), limonene (21.0 +/- 1.3%, 313.3 +/- 22.9 mg/ g EO) and myrcene (5.6 +/- 0.50 %, 65.4 +/- 7.2 mg/g EO); and the phenylpropanoid myristicin (30.6 +/- 2.3%, 438.5 +/- 40.0 mg/ g EO). The sesquiterpene germacrene D constituted 6.1 +/- 0.8%, 91.8 +/- 10.6 mg/ g EO of the oil. %26lt;br%26gt;The essential oil and the plant%26apos;s methanol and 80% methanol extracts were screened for antioxidant activity and total phenol content using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and Folin-Ciocalteu assays, respectively. The radical scavenging activity (lower IC(50)s; mu g EO or the plant material (PM) /ml 10(-4) M DPPH solution) was positively correlated to total phenolic content expressed as mg equivalents of gallic acid (EG) in 1 g EO or in the PM (IC50 633.2 +/- 0.2 mu g EO /ml DPPH; 8.5 +/- 0.2 mg EG/g EO), the methanol extracts (IC50 400.0 +/- 0.2 mu g PM /ml DPPH; 10.3 +/- 0.5 mg EG/g PM), and 80% methanol extracts (IC50 220.0 +/- 0.0 mu g PM /ml DPPH; 15.9 +/- 0.2 mg EG/g PM), respectively. The cytotoxic activity of the essential oil was measured on 3 human cancer cell lines including K562 (human chronic myelogenous leukemia), LS180 (human colon adenocarcinoma) and MCF-7 (human breast adenocarcinoma) cells. The IC50 values of the oil on K562, LS180 and MCF-7 cells were 396.8 +/- 64.9, 183.2 +/- 42.8 and 159.5 +/- 29.0 mu g/ml, respectively (mean +/- SE), while the plant extracts were not active.

• 出版日期2014-6