科研之友
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摘要
Latent tuberculosis infection (LTBI) lacks diagnostic gold method. Effective method is important to the control of tuberculosis. IFN-gamma responses in 600 military recruits were tested by ELISA using whole blood incubation with latent protein Rv2029c, Rv2659c and recombinant protein CFP10-ESAT6 (rCE) respectively. They also received tuberculin skin test. Their BCG vaccination status was recorded. When 30.7% (184/600) of recruits gave higher IFN-gamma responses (>= 470 pg/mL) to rCE as LTBI, the rests as healthy control, the AUC of rRv2029c was 0.856 and rRv2659c was 0.827 for LTBI diagnosis. IFN-gamma responses to rCE were higher in PPD-positive group (>= 5 mm) than negative group (<5 mm) (p < 0.05), while for rRv2029c and rRv2659c were not (p > 0.05). IFN-gamma responses induced by rRv2029c and rRv2659c were higher in the moderately-positive group (>= 5, <15 mm) than the strongly-positive group (>= 15 mm) (p < 0.05), while for rCE were not (p > 0.05). IFN-gamma levels to three antigens were not related to BCG vaccination status (p > 0.05). Rv2659c and Rv2029c are good candidate antigens to complement the role of rCE for LTBI diagnosis, which provide a basis for developing cost-effective LTBI screening methods in the army.
- 出版日期2017-6
- 单位中国人民解放军第三〇九医院
