Determination of Aminophylline in Plasma of Rat by UPLC Method with Ultraviolet Detection: Applications for Pharmacokinetic Studies

作者：Wang Haiyun; Gao Xiaomin; Liu Jinlai; You Weiwei; Ni Yun; Shen Xiuwei; Wu Shuzhi; Li Hao^*; Chen Lianguo^*

来源：Latin American Journal of Pharmacy, 2017, 36(12): 2451-2456.

• aminophylline
• pharmacokinetic
• plasma
• rat
• theophylline
• UPLC

A rapid, selective and sensitive analytical method based on ultra-performance liquid chromatography (UPLC) was developed and validated for the quantitative analysis of aminophylline based on the detection of theophylline in aminophylline in rat plasma. Analytes were extracted through liquid-liquid extraction, and chromatographic separation was performed on an Acquity UPLC BEH C18 column (2.1 x 50 mm, 1.7 mu m) with a water-acetonitrile mobile phase (0.2 mL/min), utilizing ultraviolet detection at 273 nm. The assay was linear over the range of 0.625-100 mu g/mL for theophylline and an excellent linearity (r = 0.9994) was achieved. The limit of quantitation (LOQ) for theophylline is 0.5 mu g/mL. Intra and inter-day precisions of theophylline were no more than 10% and its accuracies were in the range of 97.7-104.9 %. Our method was successfully applied for the determination of aminophylline in rat plasma for pharmacokinetic study.

• 出版日期2017
• 单位温州医科大学