A novel method for preparing complete antigens of gonyautoxin 2,3 and their feature of immunogenicity

作者：Zhang, Rui^*; Zhang, Caohua; Yang, XiHong; Qin, Xiaoming; Lin, Huajuan; Zhao, YunTao

来源：Toxicon, 2013, 68: 24-29.

DOI：10.1016/j.toxicon.2013.03.009

• Gonyaulax parlaytic shellfish toxins
• Artificial antigen preparation
• Immunogenicity
• UV & HPLC
• ELISA & Western-blot

In this paper, a novel method was proposed to prepare artificial antigens of gonyaulax parlaytic shellfish toxin 2 and 3 (GTX2,3). An intermediate GTX2,3-aldehyde was first synthesized by activating the NH2 group of the 2nd and 8th amino acid residues with three different aldehydes and two artificial complete antigens GTX2,3-aldehyde-bovine serum albumin (BSA) and GTX2,3-aldehyde- keyhole limpet hemocyanin (KLH) were then prepared by cross-linking the intermediate with BSA or KLH. The successful preparation of the two complete antigens was confirmed by UV spectral scanning, HPLC, production of antibodies with titer of 1.28 x 10(4) from mice immunized with the two complete antigens, indirect ELISA and Western-blot. In conclusion, the synthesized complete antigens have strong immunogenicity, which provides a solid foundation for preparing GTX2,3 monoclonal antibody and rapid detection kit.

• 出版日期2013-6-15
• 单位广东海洋大学