Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells

作者：Jiang Jia; Hao Wei; Li Yuzhuo; Yao Jinrong; Shao Zhengzhong; Li Hong; Yang Jianjun; Chen Shiyi^*

来源：Biotechnology Letters, 2013, 35(4): 657-661.

DOI：10.1007/s10529-012-1121-2

• Bone tissue engineering
• Hydroxyapatite
• Osteoconductivity
• Osteoinductivity
• Regenerated silk fibroin

A novel hydroxyapatite/regenerated silk fibroin scaffold was prepared and investigated for its potential to enhance both osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells in vitro. Approx. 12.4 +/- A 0.06 % (w/w) hydroxyapatite was deposited onto the scaffold, and cell viability and DNA content were significantly increased (18.5 +/- A 0.6 and 33 +/- A 1.2 %, respectively) compared with the hydroxyapatite scaffold after 14 days. Furthermore, alkaline phosphatase activity in the novel scaffold increased 41 +/- A 2.5 % after 14 days compared with the hydroxyapatite scaffold. The data indicate that this novel hydroxyapatite/regenerated silk fibroin scaffold has a positive effect on osteoinductivity and osteoconductivity, and may be useful for bone tissue engineering.

• 出版日期2013-4
• 单位复旦大学