Monitoring of the phenol biodegradation process with an electrochemical biosensor

作者：Boukoureshtlieva R^*; Yankova S; Beschkov V; Milusheva J; Naydenova G; Popova L; Yotov G; Hristov S

来源：Bulgarian Chemical Communications, 2013, 45: 129-134.

• biodegradation of phenol
• tyrosinase
• gas-diffusion electrode
• Klebsiella oxytoca strain

Monitoring of the water (domestic and/or industrial) pollution continuously and on regular bases is a quite severe issue, which still demands proper solution. Here we report on development of an amperometric biosensor for detection of phenol degradation in water and we compare the analytical data with those obtained from the most common analytical method: HPLC (High Performance Liquid Chromatography).
The described biosensor consists of an enzyme electrode, comprising a porous hydrophobic carbon electrode with immobilized enzyme, namely: Tyrosinase from mushroom (EC 1.14.18.1). In accordance to study the efficiency of tyrosinase amperometric sensor for the determination of phenol concentration, experiments at constant potential of 0.0V vs. Ag/AgCl reference electrode in phosphate buffer solution (pH 7.0) are performed. The response time is not exceeding 3 min and the amperometric response increased linearly up to 2 . M phenol concentration. The tyrosinase electrode exhibited a sensitivity of 330 nA . M-1 and the apparent Michaellis constant was calculated to be K-M(app) = 12.6 . M with detection limit for phenol of 0.4 . M. Moreover, the tyrosinase electrode exhibited a good reproducibility and stability during 35 days of periodical measurements.
The tyrosinase electrode investigated in our study is applied efficiently for the detection and determining of the phenol concentration in real water samples.

• 出版日期2013