A membrane fusion assay based on pore-spanning lipid bilayers

作者:Hoefer Ines; Steinem Claudia*
来源:Soft Matter, 2011, 7(5): 1644-1647.
DOI:10.1039/c0sm01429j

摘要

Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time.