AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function

作者：Venkatesh Deepak; Mruk Dolores; Herter Jan M; Cullere Xavier; Chojnacka Katarzyna; Cheng C Yan; Mayadas Tanya N^*

来源：American Journal Of Pathology, 2016, 186(2): 270-284.

DOI：10.1016/j.ajpath.2015.10.007

The blood-testis barrier (BTB), formed between adjacent Sertoli cells, undergoes extensive remodeling to facilitate the transport of preleptotene spermatocytes across the barrier from the basal to apical compartments of the seminiferous tubules for further development and maturation into spermatozoa. The actin cytoskeleton serves unique structural and supporting rotes in this process, but Little is known about the role of microtubules and their regulators during BTB restructuring. The large isoform of the cAMP-responsive scaffold protein AKAP9 regulates microtubule dynamics and nucleation at the Golgi. We found that conditional deletion of Akap9 in mice after the initial formation of the BTB at puberty leads to infertility. Akap9 deletion results in marked alterations in the organization of microtubules in Sertoli cells and a Loss of barrier integrity despite a relatively intact, albeit more apically Localized F-actin and BTB tight junctional proteins. These changes are accompanied by a Loss of haploid spermatids due to impeded meiosis. The barrier, however, progressively reseals in older Akap9 null mice, which correlates with a reduction in germ cell apoptosis and a greater incidence of meiosis. However, spermiogenesis remains defective, suggesting additional roles for AKAP9 in this process. Together, our data suggest that AKAP9 and, by inference, the regulation of the microtubule network are critical for BTB function and subsequent germ cell development during spermatogenesis.

• 出版日期2016-2