A Novel DAD Type and Folic Acid Conjugated Fluorescent Monomer as a Targeting Probe for Imaging of Folate Receptor Overexpressed Cells

作者：Kanik Fulya Ekiz; Ag Didem; Seleci Muharrem; Barlas Firat Baris; Kesik Melis; Hizalan Gonul; Akpinar Hava; Timur Suna^*; Toppare Levent

来源：Biotechnology Progress, 2014, 30(4): 952-959.

DOI：10.1002/btpr.1928

• fluorescence microscopy
• cell imaging
• folic acid
• conjugation

We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2,3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy.

• 出版日期2014-8