Fast optically sectioned fluorescence HiLo endomicroscopy

作者：Ford Tim N^*; Lim Daryl; Mertz Jerome

来源：Journal of Biomedical Optics, 2012, 17(2): 021105.

DOI：10.1117/1.JBO.17.2.021105

• endomicroscopy
• fluorescence
• optical sectioning
• fiber-bundle imaging
• double-shutter camera
• graphics processing unit
• chorioallantoic membrane

We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier demonstration of widefield optical sectioning through a flexible fiber bundle. The improved device features lateral and axial resolutions of 2.6 and 17 mu m, respectively, a net frame rate of 9.5 Hz obtained by real-time image processing with a graphics processing unit (GPU) and significantly reduced motion artifacts obtained by the use of a double-shutter camera. We demonstrate the performance of our system with optically sectioned images and videos of a fluorescently labeled chorioallantoic membrane (CAM) in the developing G. gallus embryo. HiLo endomicroscopy is a candidate technique for low-cost, high-speed clinical optical biopsies.

• 出版日期2012-2