Embryonic lethal abnormal vision-like RNA-binding proteins regulate neurite outgrowth and tau expression in PC12 cells

作者：Aranda Abreu GE; Behar L; Chung SM; Furneaux H; Ginzburg I^*

来源：Journal of Neuroscience, 1999, 19(16): 6907-6917.

• RNA-binding proteins
• tau mRNA
• mRNA stabilization
• neurite outgrowth
• antisense oligodeoxynucleotides
• microtubules

The embryonic lethal abnormal vision (ELAV)-like proteins are mRNA-binding proteins that regulate mRNA stability. The neuronal members of this family are required for neuronal differentiation. We identified the binding region of purified HuD protein to a target neuronal mRNA encoding for the tau microtubule-associated protein and demonstrated an in vivo interaction between the ELAV-like protein and its target tau mRNA. We show that treatment of neuronal cells with antisense oligode-oxynucleotides directed against HuD blocks the induction of neurite outgrowth and decreases the levels of tau mRNAs, indicating that the ELAV-like proteins are required for neuronal differentiation.

• 出版日期1999-8-15