An electrochemiluminescent biosensor for glucose based on the electrochemiluminescence of luminol on the nafion/glucose oxidase/poly(nickel(II)tetrasulfophthalocyanine)/multi-walled carbon nanotubes modified electrode

作者：Qiu Bin; Lin Zhenyu^*; Wang Jian; Chen Zhihuang; Chen Jinhua; Chen Guonan

来源：Talanta, 2009, 78(1): 76-80.

DOI：10.1016/j.talanta.2008.10.067

• Electrochemiluminescent sensor
• Glucose
• Nickel phthalocyanine
• Multi-walled carbon nanotubes
• Luminol

A poly(nickel(II) tetrasulfophthalocyanine)/multi-walled carbon nanotubes composite modified electrode (polyNiTSPc/MWNTs) was fabricated by electropolymerization of NiTSPc on MWNTs-modified glassy carbon electrode (GCE). The modified electrode was found to be able to greatly improve the emission of luminol electrochemiluminescence (ECL) in a solution containing hydrogen peroxide. Glucose oxidase (GOD) was immobilized on the surface of polyNiTSPc/MWNTs modified GC electrode by Nafion to establish an ECL glucose sensor. Under the optimum conditions, the linear response range of glucose was 1.0 x 10(-6) to 1.0 x 10(-4) mol L(-1) with a detection limit of 8.0 x 10(-1) mol L(-1) (defined as the concentration that could be detected at the signal-to-noise ratio of 3). The ECL sensor showed an outstanding well reproducibility and long-term stability. The established method has been applied to determine the glucose concentrations in real serum samples with satisfactory results.

• 出版日期2009-4-15
• 单位福州大学