UPLC-ESI-MS analysis of thymosins beta 4 and beta 10 in cell lysates: a simple, rapid and sensitive quantification method

作者:Touboul David*; Wdzieczak Bakala Joanna; Laprevote Olivier; Garcia Alvarez Maria Concepcion
来源:Analytical Methods, 2011, 3(3): 678-682.
DOI:10.1039/c0ay00576b

摘要

Fast and reproducible quantification of thymosins beta 4 and beta 10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of T beta 10 compared to control cells, whereas the level of T beta 4 is drastically depending on cell lines.

  • 出版日期2011-3

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