Detection of Hemoglobin New York [beta 113 (G15) Val -> Glu, GTG > GAG] in a Thai Woman by Capillary Electrophoresis

作者：Panyasai Sitthichai; Pornprasert Sakorn^*

来源：Indian Journal of Hematology and Blood Transfusion, 2016, 32(4): 514-516.

DOI：10.1007/s12288-016-0692-9

• Detection
• Capillary electrophoresis
• Hb New York
• High performance liquid chromatography
• Thai woman

Hemoglobin (Hb) New York [beta 113 (G15) Val -> Glu, GTG > GAG] is a very rare beta-chain variant found in Thailand. This variant is often missed by routine laboratory testing because Hb New York and Hb A have the identical retention time on high performance liquid chromatography. We reported here for the first time that the detection of Hb New York in a Thai woman by using capillary electrophoresis (CE). A peak of Hb New York located ahead of Hb A at the electrophoretic zone 11 with a level of 42.8 %. The DNA sequencing revealed the GTG > GAG mutation at codon 113 for Hb New York on one allele of beta-globin gene. Therefore, the CE has a high efficiency to prevent the misinterpretation of hemoglobin analysis in patients who are heterozygote of this variant.

• 出版日期2016-12