Two complementary approaches for intracellular delivery of exogenous enzymes

作者：Rust Aleksander; Hassan Hazirah H A; Sedelnikova Svetlana; Niranjan Dhevahi; Hautbergue Guillaume; Abbas Shaymaa A; Partridge Lynda; Rice David; Binz Thomas; Davletov Bazbek^*

来源：Scientific Reports, 2015, 5(1): 12444.

DOI：10.1038/srep12444

Intracellular delivery of biologically active proteins remains a formidable challenge in biomedical research. Here we show that biomedically relevant enzymes can be delivered into cells using a new DNA transfection reagent, lipofectamine 3000, allowing assessment of their intracellular functions. We also show that the J774.2 macrophage cell line exhibits unusual intracellular uptake of structurally and functionally distinct enzymes providing a convenient, reagent-free approach for evaluation of intracellular activities of enzymes.

• 出版日期2015-7-24