Emergence of a unique recombinant form of HIV-1 from Manipur (India)

作者：Sarkar Roni; Sarkar Kamalesh; Singh N Brajachand; Singh Y Manihar; Mitra Debashis; Chakrabarti Sekhar^*

来源：Journal of Clinical Virology, 2012, 55(3): 274-277.

DOI：10.1016/j.jcv.2012.07.012

• Injecting drug users
• Polymerase chain reaction
• Human immunodeficiency virus
• Near full-length genome

Background: The AIDS epidemic in Manipur, India, manifests unique features, having co-circulation of B and C HIV-1 subtypes along with recombinant forms. Manipur has the highest incidence of HIV-1 infection compared to the other states of India, but limited information is available regarding the full-length sequence of HIV-1 recombinants.
Objectives: To characterize the near full-length genome of a novel recombinant HIV-1 strain from an injecting drug user of Manipur.
Study design: Viral RNA, extracted from the plasma of a male injecting drug user aged 35, was diagnosed with HIV-1 infection. Near full-length genome was amplified by polymerase chain reaction using primer walking approach. Phylogenetic relationships were determined with neighbor-joining trees. The recombination break points were detected using boot scan and Simplot analyses.
Results: This recombinant predominantly had subtype C genome and exhibited mosaic structures with subtype B insertions at three different positions of HIV-1 genome. Simplot analysis of near full-length genome sequence from the recombinant HIV-1 strain, MAN86 exhibited similarity with the sequence of C.IN.93.93IN905 in its subtype C backbone, while the subtype B insertions showed resemblance with the sequence of B.TH.99.99(TH)_C1416.
Conclusions: This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from India and Thailand.

• 出版日期2012-11
• 单位常州工学院