Dried bilberry (Vaccinium myrtillus L.) extract fractions as antioxidants and cancer cell growth inhibitors

作者：Saponjac Vesna Tumbas^*; Canadanovic Brunet Jasna; Cetkovic Gordana; Djilas Sonja; Cetojevic Simin Dragana

来源：LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2015, 61(2): 615-621.

DOI：10.1016/j.lwt.2014.04.021

• Bilberry
• Vitamin C
• Phenolics
• Free radical scavenging activity
• Antiproliferative activity

Three antioxidant fractions (Fr1, Fr2 and Fr3) were separated by solid phase extraction from dried bilberry (Vaccinium myrtillus L.) and tested for antioxidant and antiproliferative activity. Vitamin C (1.02 mg/100 g) was identified in Fr1, six flavonoids in Fr2 and eight phenolic acids in Fr3. The most abundant compounds in Fr2 and Fr3 were quercetin (243.3 mg/100 g dried bilberry) and p-coumaric acid (57.87 mg/100 g dried bilberry), respectively. The best free radical scavenging activity on hydroxyl radicals was expressed by Fr3 (EC50 center dot OH = 0.117 mg/ml), and on 2,2-diphenyl-1-picrihydrazyl (DPPH) radicals by Fr2 (EC50DPPH center dot = 0.025 mg/ml). Considering the kinetic behaviour, tested by spectrophotometrical DPPH method, the highest antiradical efficiency (AE = 19.82 ml/mg x min) was demonstrated by Fr2. Fr2 and Fr3 significantly inhibited the growth of cervix epitheloid carcinoma, breast adenocarcinoma and colon adenocarcinoma cell lines, with IC50 ranging from 125.80 to 300.48 mu g/ml. Dried bilberry possesses beneficial effects in prevention of cancer and diseases which have been attributed to free radical production.

• 出版日期2015-5