Development and validation of isocratic reversed-phase HPLC method for simultaneous estimation of torsemide and spironolactone in bulk and pharmaceutical combined tablet dosage form

作者：Sharma S; Sharma M C^*; Chaturvedi S C

来源：Optoelectronics and Advanced Materials-Rapid Communications, 2010, 4(3): 427-430.

• Torsemide
• Spironolactone
• Pharmaceutical
• HPLC method

Validated high performance liquid chromatographic (HPLC) method for estimation of Torsemide (TOR) and Spironolactone (SPI) in tablet dosage form. Isocratic RP-HPLC separation was achieved on Licrosphere C(18) column (250 x 4.6mm) using Methanol: Acetonitrile: Phosphate buffer, pH 6.5 (60:20:20 v/v) at flow rate of 1.5 ml/min at 30 degrees C temperature. Quantitation was achieved by UV detection at 252 nm over the conc. range 0-25 mu g/ml for both the drugs with mean recoveries of 100.01% +/- 0.12 and 100.64% +/- 0.20 for TOR and SPI respectively. This method is simple, precise and sensitive and applicable for the simultaneous estimation of TOR and SPI in tablet pharmaceutical combined dosage form.

• 出版日期2010-3