High-performance liquid chromatography-high-resolution time-of-flight mass spectrometry in the identification and determination of penicillin and tetracycline group antibiotics in food

作者：Amelin V G^*; Korotkov A I

来源：Journal of Analytical Chemistry, 2015, 70(11): 1383-1389.

DOI：10.1134/S1061934815110027

• antibiotics of penicillin and tetracycline groups
• food products
• high-performance liquid chromatography
• high-resolution time-of-flight mass spectrometry

A method of the identification and determination of 27 antibiotics of penicillin (15) and tetracycline (12) groups in food using high-resolution time-of-flight mass spectrometry combined with high-performance liquid chromatography and a simple and fast sample preparation procedure have been developed. The analytical range for penicillins is 3 (50)-300 (1000) mu g/kg and for tetracyclines, 0.3 (1.5)-100 (200) mu g/kg. A scheme of the identification and determination of antibiotics using the standard addition method has been proposed. The relative standard deviation of the results of analysis does not exceed 10%. The time of sample screening is 30-40 min, the time of the determination of the detected antibiotics is 2-3 h.

• 出版日期2015-11