Assessment of the Probiotic Potential of a Dairy Product Fermented by Propionibacterium freudenreichii in Piglets

作者：Cousin Fabien J; Foligne Benoit; Deutsch Stephanie Marie; Massart Sebastien; Parayre Sandrine; Le Loir Yves; Boudry Gaelle; Jan Gwenael^*

来源：Journal of Agricultural and Food Chemistry, 2012, 60(32): 7917-7927.

DOI：10.1021/jf302245m

• probiotic
• dairy propionibacteria
• immunomodulation
• pig
• intestinal barrier

Dairy propionibacteria, including Propionibacterium freudenreichii, display promising probiotic properties, including immunomodulation. These properties are highly strain-dependent and rarely studied in a fermented dairy product. We screened 10 strains, grown in a newly developed fermented milk ultrafiltrate, for immunomodulatory properties in vitro. The most anti-inflammatory strain, P. freudenreichii BIA129, was further tested on piglets. P. freudenreichii-fermented product improved food intake and growth of piglets. Colonic mucosa explants of treated pigs secreted less interleukin 8 (-25%, P < 0.05) and tumor necrosis factor alpha (-20%, P < 0.05), either in basal conditions or after a lipopolysaccharide challenge. By contrast, the gut structure, barrier measured ex vivo in Ussing chambers), microbial diversity (assessed by 16S rRNA pyrosequencing), and colonic short-chain fatty acid content were unchanged, assuming maintenance of normal intestinal physiology. In conclusion, this work confirms in vivo probiotic properties of dairy propionibacteria-fermented products, which are promising for the prevention or healing of inflammatory bowel diseases.

• 出版日期2012-8-15