Dried blood spot analysis of gabapentin as a valid alternative for serum: a bridging study

作者：Sadones Nele; Van Bever Elien; Van Bortel Luc; Lambert Willy E; Stove Christophe P^*

来源：Journal of Pharmaceutical and Biomedical Analysis, 2017, 132: 72-76.

DOI：10.1016/j.jpba.2016.09.036

• Dried blood spots (DBS)
• Gabapentin
• Capillary sampling
• Bridging study
• GC-MS

We evaluated the applicability of a validated GC-MS method for the determination of gabapentin in dried blood spots (DBS). Important for the acceptance of DBS sampling as an alternative sampling strategy is the possibility to base solid conclusions on the quantification. Therefore, bridging studies-studies in which the correlation between both DBS and a reference matrix (e.g. serum) is evaluated statistically- need to be conducted. To this end, a comparative study was set up to quantify gabapentin in both blood (DBS) and serum samples. Statistically significant differences between DBS and serum concentrations were found (p<0.001). A mean blood-to-serum ratio of 0.85 was observed, which is in line with expectations. Calculated serum concentrations (obtained by dividing the DBS concentrations by 0.85) demonstrated a good correlation with measured serum concentrations, with 87% of samples fulfilling the criterion for incurred sample reanalysis. Furthermore, our data indicate a good correlation between capillary and venous concentrations. Conclusively, this study demonstrated that DBS are a valid alternative to serum for the determination of gabapentin.

• 出版日期2017-1-5