青海云杉ISSR-PCR反应体系的建立与优化(英文)

作者：王文峰; 李毅; 马彦军; 李栋栋

来源：Agricultural Science & Technology, 2009, 10(03): 68-71.

DOI：10.16175/j.cnki.1009-4229.2009.03.024

• Picea crassifolia kom
• ISSR
• System establishment
• Optimization Picea crassifolia kom
• Optimization

[Objective] The experiment aimed to determine the optimum ISSR-PCR reaction system of Picea crassifolia kom. [Method] Picea crassifolia kom. was used as material to select and optimize influencing factors of ISSR-PCR such as Mg2+, dNTPs, Taq DNA polymerase, template DNA, primers, annealing temperatu...

• 出版日期2009
• 单位甘肃农业大学