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摘要
Objective: To study the modulatory effects and mechanism of GABA(B)R on the cognitive function of epileptic rats induced by pilocarpine. Method: The lithium chloride (LiCl)-pilocarpine induced epileptic model was established in SD rats, and those rats were randomly divided into GABA(B)R groups (saline group, Baclofen group, CGP group) and Arc groups (Sham operation group, Empty vector group, Virus group). Morris water-maze test and passive avoidance performance test were used to detect the cognition of epileptic rats. The expression of GABA(B)R (GB(1), GB(2)), and Arc/Arg3.1 in hippocampus of epileptic rats were determined by immunohistochemistry and Western Blotting at protein level. In addition, the expression of GABA(B)R (GB(1), GB(2)), and Arc/Arg3.1 was detected by RT-PCR at mRNA level. Results: The results of Morris water-maze test and passive avoidance performance test showed that the learning ability and cognitive performance improved in the rats of CGP group but declined in Baclofen group and Virus group, comparing with the control. The data from immunohistochemistry, RT-PCR and Western blotting revealed that the relative expression of Arc/Arg3.1 was significantly increased in CGP group comparing with the other two groups but decreased in Baclofen group and virus group comparing with the other two groups (P<0.05). The relative expression ofGB(1) and GB(2) was significantly increased comparing with the other two groups but had no significant difference between virus group and the other two groups. Conclusion: GABA(B)R influenced the spatial learning and memory of epileptic rats by regulating Arc/Arg3.1.
- 出版日期2016
- 单位宁夏医科大学
