Determination of biapenem in a medicinal product by micellar electrokinetic chromatography with sweeping in an enhanced electric field

作者：Michalska Katarzyna^*; Cielecka Piontek Judyta; Pajchel Genowefa; Tyski Stefan

来源：Journal of Chromatography A, 2013, 1282: 153-160.

DOI：10.1016/j.chroma.2013.01.042

• Antibiotics
• CE
• Biapenem
• Pharmaceutical analysis

The elaborated method of micellar electrokinetic chromatography (MEKC) used to separate biapenem from its related substances was successfully implemented using sweeping under an enhanced electric field, followed by UV absorption detection at 200 nm. The best results were obtained with formic buffer (22.5 mM) pH 4.3 and sodium dodecyl sulfate (150 mM) added to the electrolyte as the sweeping agent. Neutral capillary (60/50 cm; 501 mu m ID) with reverse polarity and voltage values of 22 kV, were used throughout the investigation. %26lt;br%26gt;The optimized method of biapenem determination, validated in terms of linearity, accuracy and precision, provides a detection limit of 0.5 mu g/mL at S/N = 3 for biapenem. The repeatability of the CE system, expressed by relative standard deviations (RSD) in the migration times, for biapenem and its degradation products varied from 0.14 to 1.48%, whereas for the corrected peak areas RSD were about 0.68-8.43%. Satisfactory separation was achieved within 20 min of electrophoresis; moreover all carbapenems (imipenem, meropenem, ertapenem, doripenem and biapenem) were separated from each other during analysis. The evaluated MEKC method was applied to the analysis of a medicinal product containing biapenem - Omegacin (R) 0.3 g for intravenous drip infusion.

• 出版日期2013-3-22