Antioxidant activity and inhibition effect on the growth of human colon carcinoma (HT-29) cells of esculetin from Cortex Fraxini

作者：Wang, Kai; Zhang, Ye; Ekunwe, S. I. N.; Yi, Xianghui; Liu, Xianxian; Wang, Hengshan; Pan, Yingming^*

来源：Medicinal Chemistry Research, 2011, 20(7): 968-974.

DOI：10.1007/s00044-010-9426-y

• Esculetin
• Antioxidant
• HT-29 cell
• Thymidine incorporation

Esculetin was isolated from Cortex fraxini and identified by UV, IR, H-1 NMR, C-13 NMR. Its antioxidant properties were investigated employing various in vitro assays, such as 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, hydroxyl radical scavenging assay in a new resonance scattering (RS) method, reducing power and total antioxidant assay. Esculetin showed excellent antioxidant properties superior to synthetic antioxidant butylated hydroxytoluene (BHT). Subsequently, inhibition effect of esculetin on human colon carcinoma (HT-29) cells proliferation was measured by thymidine incorporation assay. HT-29 cell number was decreasing with increasing concentration of esculetin at the lower concentrations of 0.25, 0.5, 1.0, and 2.0 mg/ml, compared to the positive control and HT-29 cells were stimulated in response to treatment with esculetin which decreases in a dose-dependent manner. However, at the higher concentrations of 4.0, 8.0, 12.0 and 16.0 mg/ml, growth of HT-29 cells were inhibited in a concentration-dependent fashion.

• 出版日期2011-9
• 单位桂林理工大学; 广西师范大学