A Glucose Sensor Fabricated by Piezoelectric Inkjet Printing of Conducting Polymers and Bienzymes

作者：Yun Yeon Hee; Lee Byung Kook; Choi Ji Suk; Kim Sungwon; Yoo Bongyoung; Kim Yong Shin; Park Kinam; Cho Yong Woo^*

来源：Analytical Sciences, 2011, 27(4): 375-379.

Piezoelectric inkjet printing of polymers and proteins holds great promise for fabrication of miniaturized bioelectronic devices, such as biochips and biosensors. In this study, a bienzymatic glucose biosensor prototype based on poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid) (PEDOT-PSS), glucose oxidase (GOD), and horseradish peroxidase (HRP) was fabricated by a piezoelectric inkjet printer. An aqueous bioelectrical ink containing PEDOT-PSS, GOD, and HRP was prepared and printed on an indium-tin-oxide (ITO)-coated poly(ethylene terephthalate) (PET) film. The PEDOT-PSS/GOD/HRP sensor was covered with a cellulose acetate membrane. The use of bienzymatic sensing combined with conducting polymers via piezoelectric inkjet printing showed a synergistic effect resulting in significant amplification of the response signal. The glucose sensor reached steady-state current density within 3 s, indicating a fast response time, and exhibited a linear dose-dependent electrochemical response with high sensitivity. The overall result demonstrates that a glucose sensor with high sensitivity could be readily fabricated by a piezoelectric inkjet printing system.

• 出版日期2011-4