Negative electron transfer dissociation Fourier transform mass spectrometry of glycosaminoglycan carbohydrates

作者：Leach Franklin E III^*; Wolff Jeremy J; Xiao Zhongping; Ly Mellisa; Laremore Tatiana N; Arungundram Sailaja; Al Mafraji Kanar; Venot Andre; Boons Geert Jan; Linhardt Robert J; Amster I Jonathan

来源：European Journal of Mass Spectrometry, 2011, 17(2): 167-176.

DOI：10.1255/ejms.1120

• glycosaminoglycans
• Fourier transform mass spectrometry
• electrospray ionization
• negative electron detachment dissociation
• ion-ion reactions
• electron detachment dissociation
• heparan sulfate
• dermatan sulfate
• heparin

Electron transfer through gas-phase ion-ion reactions has led to the widespread application of electron-based techniques once only capable in ion trapping mass spectrometers. Although any mass analyzer can, in theory, be coupled to an ion-ion reaction device (typically a 3-D ion trap), some systems of interest exceed the capabilities of most mass spectrometers. This case is particularly true in the structural characterization of glycosaminoglycan (GAG) oligosaccharides. To adequately characterize highly sulfated GAGs or oligosaccharides above the tetrasaccharide level, a high-resolution mass analyzer is required. To extend previous efforts on an ion trap mass spectrometer, negative electron transfer dissociation coupled with a Fourier transform ion cyclotron resonance mass spectrometer has been applied to increasingly sulfated heparan sulfate and heparin tetrasaccharides as well as a dermatan sulfate octasaccharide. Results similar to those obtained by electron detachment dissociation are observed.

• 出版日期2011
• 单位中国海洋大学