In vitro toxicity studies of zinc oxide nano- and microrods on mammalian cells: A comparative analysis

作者：Zanni Elena; De Palma Silvia; Chandraiahgari Chandrakanth Reddy; De Bellis Giovanni; Cialfi Samantha; Talora Claudio; Palleschi Claudio; Sarto Maria Sabrina; Uccelletti Daniela^*; Mancini Patrizia^*

来源：Materials Letters, 2016, 179: 90-94.

DOI：10.1016/j.matlet.2016.05.067

• Zinc oxide
• ZnO nanorods
• ZnO microrods
• HaCaT cells
• MCF7 cells
• Cytotoxicity

This study aims at investigating the cytotoxicity of zinc oxide nanorods (ZnO-NR) and microrods (ZnO-MR) in two different human cell lines. The materials were produced by a simple and cost-effective hydrothermal route and induced a slight dose dependent toxic effect on MCF7 and HaCaT cells. Both cell lines showed a very low cytotoxicity when exposed to suspensions containing 1-100 mu g/ml of ZnO-NR and ZnO-MR. Moreover, Field-emission scanning electron microscopy (FE-SEM) images of both cell lines exposed to the materials showed no significant effect on cell morphology compared to untreated cells. However, the treatments induced modifications of filopodia, mainly in MCF7 cells exposed to ZnO-NR. In addition, a very slight increase in ROS generation was observed only in ZnO-MR-treated cells. Moreover, an apoptotic effect of ZnO-NR rather than a cell cycle arrest was observed in MCF7 cells after treatment.
Overall the cytotoxicity in in vitro systems reflects a biocompatibility of both ZnO-NR and ZnO-MR, representing powerful tools in several applications.

• 出版日期2016-9-15