Amperometric nitrite biosensor based on a gold electrode modified with cytochrome c on Nafion and Cu-Mg-Al layered double hydroxides

作者：Yin, Huanshun; Zhou, Yunlei; Liu, Tao; Cui, Lin; Ai, Shiyun^*; Qiu, Yanyan; Zhu, Lusheng

来源：Microchimica Acta, 2010, 171(3-4): 385-392.

DOI：10.1007/s00604-010-0444-8

• Cu-Mg-Al layered double hydroxide
• Nafion
• Cytochrome c
• Nitrite biosensor
• Direct electrochemistry
• Determination

An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transfom infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 mu M. The detection limit is 2x10(-7) M (S/N=3). The biosensor was successfully applied to the determination of nitrite in food samples.

• 出版日期2010-12
• 单位山东农业大学; 北京师范大学