Determination of Cefprozil in Human Plasma Using High Performance Liquid Chromatography and its Application to Bioequivalence Study

作者：He, Ying-bo; Zhang, Chun-xia; Pan, Xiao-feng; Du, Xiao-xiang^*

来源：Latin American Journal of Pharmacy, 2014, 33(2): 288-293.

• Bioequivalence
• Cefprozil
• HPLC
• Plasma

In this study, a simple, rapid and sensitive high performance liquid chromatography (HPLC) method is described for determination of cefprozil in plasma samples from bioequivalence assay. Sample preparation was accomplished through protein precipitation with 35% perchloric acid, and chromatographic separation was performed on a ZORBAX Eclipse XDB-C8 (4.6x75 mm, 33 pm) at 30 C. Mobile phase consisted of a mixture of acetonitrile-0.2% trifluoroacetic acid in water-water (15:40:45) at flow rate of 1.0 mL/min. Wavelength was set at 282 nm. The method was applied to a bioequivalence study of two drug products containing cefprozil, and allowed determination of cefprozil with a higher throughput than previously described methods.

• 出版日期2014-3
• 单位温州医科大学