Analysis and three-dimensional visualization of collagen in artificial scaffolds using nonlinear microscopy techniques

作者：Filova Eva; Burdikova Zuzana; Rampichova Michala; Bianchini Paolo; Capek Martin; Kostakova Eva; Amler Evzen; Kubinova Lucie^*

来源：Journal of Biomedical Optics, 2010, 15(6): 066011.

DOI：10.1117/1.3509112

• second-harmonic generation imaging
• multiphoton microscopy
• tissue engineering
• artificial scaffolds
• collagen

Extracellularly distributed collagen and chondrocytes seeded in gelatine and poly-epsilon-caprolactone scaffolds are visualized by two-photon excitation microscopy (TPEM) and second-harmonic generation (SHG) imaging in both forward and backward nondescanned modes. Joint application of TPEM and SHG imaging in combination with stereological measurements of collagen enables us not only to take high-resolution 3-D images, but also to quantitatively analyze the collagen volume and a spatial arrangement of cell-collagen-scaffold systems, which was previously impossible. This novel approach represents a powerful tool for the analysis of collagen-containing scaffolds with applications in cartilage tissue engineering.

• 出版日期2010-12