Understanding new paradigm of plant metabolomes through secondary metabolite enrichment

作者：Aakanksha Wany^*; Nigam V K; Pandey D M

来源：Research Journal of Biotechnology, 2014, 9(4): 90-98.

• Data-mining tools
• High throughput screening systems
• In-silico analysis
• plant cell culture
• secondary metabolites

Recent achievements in the metabolic engineering of plant secondary metabolism have helped to modulate various pathways using genes encoding biosynthetic enzymes or genes encoding regulatory proteins. To increase secondary metabolite production, different strategies such as the metabolic reactions in a pathway should overcome rate limiting steps, reduce catabolism and over-expression of regulatory genes can be pursued. New functional compounds can be discovered by using high throughput systems. The metabolic pathways and its genes involved can be determined by studying functional genomics and computational approaches. In silico analysis and docking studies of inhibitors that regulate enzymes and their role in secondary metabolite enrichment have been already elucidated.
Functional genomics approaches are powerful tools to accelerate comprehensive investigations of cellular metabolism in specialized tissues or whole organisms. The technology of large-scale plant cell culture is feasible for the industrial production of plant-derived fine chemicals but economically there is low productivity of desired compounds. In recent years, metabolic engineering has opened new promising perspectives for improved production of secondary metabolites in a plant or plant cell culture and find applications in pharmaceuticals, insecticides, dyes and perfumery.

• 出版日期2014-4