Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal N-15(2)-diazirine molecular tags

作者：Theis Thomas; Ortiz Gerardo X Jr; Logan Angus W J; Claytor Kevin E; Feng Yesu; Huhn William P; Blum Volker; Malcolmson Steven J; Chekmenev Eduard Y; Wang Qiu; Warren Warren S

来源：Science Advances, 2016, 2(3): e1501438.

DOI：10.1126/sciadv.1501438

Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize N-15(2) magnetization and long-lived N-15(2) singlet spin order, with signal decay time constants of 5.8 and 23 min, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. N-15(2)-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.

• 出版日期2016-3