Using multiple quantum coherence to increase the N-15 resolution in a three-dimensional TROSY HNCO experiment for accurate PRE and RDC measurements

作者：Hu Kaifeng; Doucleff Michaeleen; Clore G Marius^*

来源：Journal of Magnetic Resonance, 2009, 200(2): 173-177.

DOI：10.1016/j.jmr.2009.06.019

• TROSY HNCO
• High resolution
• RDC
• PRE

We present a new version of the 3D TROSY HNCO pulse scheme, referred to as HR-TROSY HNCO, with comparable resolution in the N-15 dimension to a 2D H-1-N-15 HSQC experiment. In the conventional 3D TROSY HNCO, the constant time period (1/2J(NC) similar to 32 ms) severely limits the maximum resolution in the N-15 dimension. In the HR-TROSY HNCO experiment presented here, both constant time periods (similar to 32 ms each) for coherence forward and backward transfer between N-15 and C-13' are utilized to double the N-15 evolution time. This leads to a dramatic enhancement in peak separation along the N-15 dimension, making the HR-TROSY HNCO an ideal pulse scheme for accurate paramagnetic relaxation enhancement and residual dipolar coupling measurements. Published by Elsevier Inc.

• 出版日期2009-10
• 单位NIH